主持科研项目（2018年至今）
1.国家自然科学基金面上项目：脊尾白虾MSTN基因缺失致死的分子机制及其基因编辑育种的理论基础（32373121），2024.01-2027.12；直接经费50万元，张继泉主持
2.国家自然科学基金面上项目：脊尾白虾内生蛋白SRα介导的基因编辑技术及其普适性研究（32172954），2022.01-2025.12；直接经费58万元，张继泉主持
3.国家自然科学基金面上项目：基于CRISPR/Cas9技术研究脊尾白虾类胡萝卜素氧化酶基因的功能及其育种价值评估（31872613）；2019.01-2022.12；直接经费62万元，张继泉主持
4.国家自然科学基金面上项目：脊尾白虾胞苷和腺苷脱氨酶作为单碱基基因编辑工具酶的可行性研究（41876196）；2019.01-2022.12；直接经费62万元，孙玉英主持
5.国家重点研发计划“蓝色粮仓科技创新”项目子课题：水产养殖生物性别和发育的分子基础与调控机制（2018YFD0900205-7）；2018-2022；72.82万元，张继泉主持
6.河北省重点研发项目：经济甲壳动物基因编辑技术体系的建立及其在脊尾白虾分子设计育种中的应用（22323201D）；2022.07-2025.06；30万元，张继泉主持
7.河北省自然科学基金面上项目：中华锯齿米虾基因编辑平台的建立及其在分子设计育种中的应用（C2019201236）；2019.01-2021.12；10万元，张继泉主持
8.河北省自然科学基金面上项目：脊尾白虾卵黄蛋白结合蛋白携带外源蛋白从体液导入受精卵的可行性研究（D2019201239）；2019.01-2021.12；10万元，孙玉英主持
9.河北省自然科学基金面上项目：基于基因组学与基因编辑探究甲壳蓝蛋白调控甲壳动物体色的分子机制（D2022201003）；2022.01-2024.12；10万元，孙玉英主持
10.河北省自然科学基金面上项目：基于因编辑技术探究dmrt基因对甲壳动物的性别调控（D2023201002）；2023.01-2025.12；6万元，张继泉主持
11.河北省教育厅重点项目：甲壳动物蜕皮级联信号通路的解析（ZD2022093）；2022.01-2024.12；7.5万元，张继泉主持
12.河北大学多学科交叉项目：中华锯齿米虾基因组解析及分子设计育种平台建立（DXK201909），2019.01-2022.12；20万元，张继泉主持
13.河北大学科研项目：甲壳动物性别决定于性别分化的分子机制（521000981187-2)，2023.05-2026.04；10万元，张继泉主持
14.横向课题：基于CRISPR/Cas9的虾蟹类基因编辑方法研究；2021.01-2022.12；2万元，张继泉主持
论文发表（2018年至今）
2024年
35.Yan C, Liu Y, Wu Z,Sun Y*（孙玉英）,Zhang J*（张继泉）(2024)Comparative transcriptomic analysis primarily explores the molecular mechanism of compound eye formation inNeocaridina denticulata sinensis. BMC Genomics 25: 570.Doi: 10.1186/s12864-024-10453-5.
34.Wu Z, Yan C, Xing K, Liu Y, Zhang C, Li H,Sun Y*（孙玉英）,Zhang J*（张继泉）(2024)Membrane-bound trehalase enhances cadmium tolerance by regulating cell apoptosis inNeocaridina denticulata sinensis. Science of The Total Environment,Doi: 10.1016/j.scitotenv.2024.173798.
33.Gao X, Duan M,Sun Y*（孙玉英）,Zhang J*（张继泉）(2024)Molecular cloning and functional analysis of two calcium-associated cuticular protein genes inNeocaridina denticulata sinensis. Journal of Oceanology and Limnology,Doi: 10.1007/s00343-024-3211-0.
32.Xing K, Li H, Wang X,Sun Y*（孙玉英）,Zhang J*（张继泉）(2024)A Full-Length Transcriptome and Analysis of the NHL-1 Gene Family inNeocaridina denticulata sinensis. Biology13(6): 366.
31. Meng T, Ren Y, Wang Q, Lu L, Luo Y,Zhang J（张继泉）, Zhang Q, Dzantiev B, Negahdary M, Wan Y, Sun Y*, Song F*(2024) CRISPR-Cas14a with competitive isothermal amplification for rapid visual pathogen diagnosis. Sensors and Actuators B-Chemical 400: 134946.
2023年
30. Xu C, Wang Y, Zhang R,Zhang J*（张继泉）,Sun Y*（孙玉英）(2023) Molecular characterization and functional analysis of peroxiredoxin 3 (NdPrx3) fromNeocaridina denticulata sinensis. Fish & Shellfish Immunology Reports 4: 100081.
29.Zhang Z（张泽文）, Li X, Zou G, Liang H*(2023) Molecular characterization and response of silver carp (Hypophthalmichthys molitrix) under hypoxia stress. Fishes-Basel 8, 8080425.
28. Yan C, Xing K, Liu Y, Kong W, Zhang R,Sun Y*（孙玉英）,Zhang J*（张继泉）(2023) Genome-wide identification and expression profiling of Wnt gene family inNeocaridina denticulata sinensis. Gene 854: 147122.
27 Chen X, Yang H, Ruan Y, Zhou M, Liu J, Li Z, Wu X, Ren C, Zhang X,Zhang J（张继泉）, Hu C, Li H, Li G, Zhu C*, Chen T*(2023) Pacific white shrimp (Litopenaeus vannamei) vitelline membrane outer layer protein 1 (VMO1) is produced in the hepatopancreas and transported into ovarian oocytes during vitellogenesis. Gene 851: 147027.
2022年
26. Feng D, Gao X, Kong W, Wu Z, Yan C, Liu Y, Xing K,Sun Y*（孙玉英）,Zhang J*（张继泉）(2022) An extracellular Cu/Zn superoxide dismutase fromNeocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein. Fish & Shellfish Immunology 128: 547-556.
25. Kong W, Wu Z, Liu Y, Yan C,Zhang J*（张继泉）,Sun Y*（孙玉英）(2022) RNA-seq analysis revealing the immune response ofNeocaridina denticulata sinensisgill toVibrio parahaemolyticusinfection. Fish & Shellfish Immunology 130: 409-417.
24. Liang M, Ma L, Li X, Feng D,Zhang J*（张继泉）,Sun Y*（孙玉英）(2022) Identification and characterization of two types of triacylglycerol lipase genes fromNeocaridina denticulata sinensis. Fish & Shellfish Immunology 131: 980-990.
23. Liu M, Wu Z, Yan C, Liu Y, Xing K,Zhang J*（张继泉）,Sun Y*（孙玉英）(2022) Ovarian transcriptome and metabolic responses of RNAi-mediated farnesyl pyrophosphate synthase knockdown inNeocaridina denticulata sinensis. Genomics 114(6): 110484.
22. Liu M, Yan C, Liu Y, Wu Z,Zhang J*（张继泉）,Sun Y*（孙玉英）(2022) Cloning, expression analysis and RNAi of farnesoic acid O-methylransferase gene fromNeocaridina denticulata sinensis. Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology 259: 110719.
21. Liu Y, Wu Z, Guo K, Zhou Y, Xing K, Zheng J,Sun Y*（孙玉英）,Zhang J*（张继泉）(2022) Metallothionein-1 gene fromExopalaemon carinicaudaand its response to heavy metal ions challenge. Marine Pollution Bulletin 175: 113324.
20. Liu Y, Xing K, Yan C, Zhou Y, Xu X,Sun Y*（孙玉英）,Zhang J*（张继泉）(2022) Transcriptome analysis of Neocaridina denticulate sinensis challenged byVibrio parahemolyticus. Fish & Shellfish Immunology 121: 31-38.
19. Liu Y, Xing K, Wu Z, Yan C,Sun Y*（孙玉英）,Zhang J*（张继泉）(2022) Construction of a cytidine base editor based on cytidine deaminase and its application in crustacean genome editing. Aquaculture Reports 27: 101366
18. Wang Y, Zhang R, Xu C,Sun Y*（孙玉英）,Zhang J*（张继泉）(2022) Characterization and functional analysis of peroxiredoxin 4 gene in theNeocaridina denticulata sinensis. Fish & Shellfish Immunology 122: 162-169.
17. Xing K, Liu Y, Yan C, Zhou Y, Zhang R,Sun Y*（孙玉英）,Zhang J*（张继泉）(2022) Transcriptomic analysis ofNeocaridina denticulate sinensishepatopancreas indicates immune changes after copper exposure. Fish & Shellfish Immunology 121: 23-30.
16. Yang H, Chen X, Li Z, Wu X, Zhou M, Zhang X, Liu Y,Sun Y（孙玉英）, Zhu C, Guo Q, Chen T*,Zhang J*（张继泉）(2022) Genome-Wide analysis indicates a complete prostaglandin pathway from synthesis to inactivation in Pacific white shrimp,Litopenaeus vannamei. International Journal of Molecular Sciences 23(3). 23031654.
15. Zhang R, Wang Y, Xu C, Chen F, Yu Q,Sun Y*（孙玉英）,Zhang J*（张继泉）(2022) Characterization of peroxiredoxin fromNeocaridina denticulata sinensisand its antioxidant and DNA protection activity analysis. Fish & Shellfish Immunology 127: 211-218.
14. Zhou Y, Song Q, Liu Y,Sun Y*（孙玉英）,Zhang J*（张继泉）(2022) A novel type I Crustin fromExopalaemon carinicauda: Antimicrobial ability related to conserved cysteine. Fish & Shellfish Immunology 127: 948-955.
2021年
13. Wu Z, Liu Y, Zheng J, Zhou Y, Xing K,Sun Y*（孙玉英）,Zhang J*（张继泉）(2021) Genomic structure, expression and functional characterization of arginine kinase (EcAK) fromExopalaemon carinicauda. Fish & Shellfish Immunology 109: 82-86.
12. Xing K, Liu Y, Yan C, Zhou Y,Sun Y（孙玉英）, Su N, Yang F, Xie S*,Zhang J*（张继泉）(2021) Transcriptome analysis ofNeocaridina denticulate sinensisunder copper exposure. Gene 764: 145098.
11. Zhang X, Yang H, Li H, Chen T, Ruan Y, Ren C, Luo P, Wang Y, Liu B, Li H, Zhong P,Zhang J（张继泉）, Jiang X, Hu C*(2021) Molecular identification of anion exchange protein 3 in Pacific white shrimp (Litopenaeus vannamei): mRNA Profiles for tissues, ontogeny, molting, and ovarian development and its potential role in stress-induced gill damage. Frontiers in Physiology 12: 726600.
2020年
10.Sun Y（孙玉英）, Liu M, Yan C, Yang H, Wu Z, Liu Y, Su N, Hou J, Zhang J, Yang F,Zhang J*（张继泉）(2020) CRISPR/Cas9-mediated deletion of beta, beta-carotene 9', 10'-oxygenase gene (EcBCO2) fromExopalaemon carinicauda. International Journal of Biological Macromolecules 151: 168-177.
9.Sun Y（孙玉英）, Yan C, Liu M, Liu Y, Wang W, Cheng W, Yang F,Zhang J*（张继泉）(2020) CRISPR/Cas9-mediated deletion of one carotenoid isomerooxygenase gene (EcNinaB-X1) fromExopalaemon carinicauda. Fish & Shellfish Immunology 97: 421-431.
2019年
8.Zhang J（张继泉）, Liu Y, Zhou Y, Wang W, Su N,Sun Y*（孙玉英）(2019) Cloning of a trehalose-6-phosphate synthase gene fromExopalaemon carinicaudaand its expression response to bacteria challenge. Fish & Shellfish Immunology 93: 387-394.
7. Zhang X#, Yuan J#, Sun Y#, Li S#, Gao Y#, Yu Y#, Liu C, Wang Q, Lv X, Zhang X, Ma KY, Wang X, Lin W, Wang L, Zhu X, Zhang C,Zhang J（张继泉）, Jin S, Yu K, Kong J, Xu P, Chen J, Zhang H, Sorgeloos P, Sagi A, Alcivar-Warren A, Liu Z, Wang L, Ruan J, Chu KH*, Liu B*, Li F*, Xiang J*(2019) Penaeid shrimp genome provides insights into benthic adaptation and frequent molting. Nature Communications 10(1): 356.
2018年
6.Zhang J（张继泉）, Song F,Sun Y*（孙玉英）, Yu K, Xiang J (2018) CRISPR/Cas9-mediated deletion ofEcMIHshortens metamorphosis time from mysis larva to postlarva ofExopalaemon carinicauda. Fish & Shellfish Immunology 77: 244-251.
5.Sun Y（孙玉英）,Zhang J*（张继泉）, Song F, Wang J, Zhang Z, Xiang J (2018) Enzymatic characterization and functional analysis of EcChi3C from ridgetail white prawnExopalaemon carinicauda. International Journal of Biological Macromolecules 109: 448-456.
4.Sun Y（孙玉英）,Zhang J*, Xiang J (2018) Immune function against bacteria of chitin deacetylase 1 (EcCDA1) fromExopalaemon carinicauda. Fish & Shellfish Immunology 75: 115-123.
3.Sun Y（孙玉英）,Zhang J*（张继泉）, Xiang J (2018) Molecular characterization and function of beta-N-acetylglucosaminidase from ridgetail white prawnExopalaemon carinicauda. Gene 648: 12-20.
2.Zhang J（张继泉）, Liu Y, Li Y, Su N, Zhou Y, Xiang J,Sun Y*（孙玉英）(2018) Biological function of a gC1qR homolog (EcgC1qR) ofExopalaemon carinicaudain defending bacteria challenge. Fish & Shellfish Immunology 82: 378-385.
1. Chen T, Lin T, Li H, Lu T, Li J, Huang W, Sun H, Jiang X,Zhang J（张继泉）, Yan A, Hu C, Luo P, Ren C*(2018) Heat shock protein 40 (hsp40) in Pacific white shrimp (Litopenaeus vannamei): Molecular cloning, tissue distribution and ontogeny, response to temperature, acidity/alkalinity and salinity stresses, and potential role in ovarian development. Frontiers in Physiology 9: 1784.
授权国家发明专利
1.中国明对虾抗脂多糖因子LBD结构域突变体文库及其构建方法和应用.发明人：张继泉,宋凤阁,相建海.专利号: ZL201610506180.X
2.一种脊尾白虾卵黄蛋白结合蛋白基因及其应用.发明人：张继泉,邢珂凡,刘玉洁,孙玉英.专利号: ZL201911421599.5
3.基于脊尾白虾胞苷脱氨酶的单碱基基因编辑系统及其构建和应用。发明人：张继泉,刘玉洁,邢珂凡,孙玉英.专利号: ZL201911421397.0
科研教学奖励
1. 2019年河北省自然科学三等奖（张继泉排名第1，孙玉英排名第4）
2. 2018年海洋科学技术（研究类）二等奖（张继泉排名第6）
3.杭州钱江特聘专家-虾蟹类育种高级顾问（张继泉，杭州市人力资源和社会保障局，2019年-）
4. 2021年全国大学生生命科学竞赛国家级二等奖/河北省一等奖1项（指导教师：张继泉）。
